Practical Biochemistry (Paperback)  | Released: 12 Jun 2016

The contents of the book have been organized to facilitate the learning process and preparation for the practical examination. Contains well-illustrated real images of tests and instruments helpful to recollect and retain the concepts easily.Organizes in a practical examination-oriented format including different sections such as:Section on Qualitative Analysis- To provide comprehensive analysis of different tests in order to impart a grasp of concepts to learners. Section on Quantitative Analysis: To enable the students to master estimation experiments. The various aspects of them are different methods, reference ranges and interpretations. Section on Charts- To improve critical thinking, an essential quality for any doctor or scientist. This section contains 54 clinically-oriented questions with answers to reinforce the information tested by the questions. Section on Spotters- To sharpen the abruptness, a skill to be attained by a medical professional. More than 100 spotters are included to serve this purpose. Section on Objective Structured Practical Examination (OSPE) Questions- To improve the clarity of practical work, this section also contains model OSPE questions for practice. This section will definitely boost the confidence of students to face OSPE.Questions are provided in every chapter for better comprehension.Useful for daily practical work of students as it helps to carry out experiments, recognize the errors during experimentation, improve practical skills, sharpen the observation ability, develop interpretation capacity to enable biochemical diagnosis of different clinical conditions, and perform brilliantly in practical examinations.Most of the diagrammatic figures are replaced by real images in Qualitative Analysis and Spotters sections. Assists faculty and auxiliary staff in setting up laboratory for practical work as the details of reagent preparations are readily accessible with each chapter.The book is primarily written for medical students, but it should also be useful to students of dentistry and pharmacy. About the AuthorGeetha Damodaran K MBBS MD (Biochemistry)Table of Contents: Contents SECTION 1: QUALITATIVE ANALYSIS 1. Reactions of Carbohydrates 3 Classification 3 Reactions of Monosaccharides 4 Reactions of Disaccharides 11 Reactions of Polysaccharides 15 Reactions of Starch 15 Identification of Unknown Carbohydrates 17 Questions 18 Reagent Preparation 18 2. Reactions of Proteins 19 Precipitation Reactions of Proteins 19 Color Reactions of Proteins and Amino Acids 25 Specific Color Reactions used to Identify the Side Chain (R) Groups of Amino Acids 27 Reactions of Albumin 32 Reactions of Casein 32 Identification of Unknown Proteins 34 Reactions of Gelatin 34 Questions 35 Reagent Preparation 35 3. Reactions of Lipids 37 General Reactions of Lipids 37 Reactions of Fats and Fatty Acids 37 Cholesterol 39 Questions 41 Reagent Preparation 41 4. Reactions of Urea 42 General Reactions of Urea 42 Questions 44 Reagent Preparation 44 5. Reactions of Creatinine 45 General Reactions of Creatinine 45 Questions 46 Reagent Preparation 46 Prelims.indd 13 17-07-2015 14:15:34 xiv Practical Biochemistry 6. Reactions of Uric Acid 47 General Reactions of Uric Acid 47 Questions 49 Reagent Preparation 49 7. Schemes for Identification of Biologically Important Compounds 50 For Biologically Important Carbohydrate Substances Comprising Glucose, Fructose, Sucrose, Lactose, Maltose 50 For Biologically Important Substances Comprising Glucose, Fructose, Sucrose, Lactose, Maltose, Albumin, Urea, Uric Acid and Creatinine 51 For Biologically Important Substances Comprising Glucose, Fructose, Sucrose, Lactose, Maltose, Albumin, Casein, Urea, Uric Acid and Creatinine 52 8. Urine Analysis 53 Analysis of Normal Constituents of Urine 53 Analysis of Abnormal Constituents of Urine 60 Questions 66 Reagent Preparation 66 9. Identification of Hemoglobin Derivatives 67 Spectroscopic Examination of Hemoglobin Pigments 67 Identification of Hemoglobin Pigment by Microscopy 71 Questions 72 Reagent Preparation 72 10. Reactions of Milk 74 Precipitation of Casein from Milk 74 Questions 75 Reagent Preparation 75 SECTION 2: QUANTITATIVE ANALYSIS 11. Introduction to Quantitative Analysis 79 Principles of Colorimetry 79 Components of Photoelectric Colorimeter 83 Measurement in a Photoelectric Colorimeter 83 Questions 84 12. Determination of Glucose 85 Determination of Glucose Concentration 85 Glucose Tolerance Test 88 Questions 91 Reagent Preparation 91 13. Determination of Urea 93 Urea 93 Questions 96 Reagent Preparation 96 Prelims.indd 14 17-07-2015 14:15:34 Contents xv 14. Determination of Creatinine 98 Creatinine 98 Questions 101 Reagent Preparation 101 15. Determination of Total Protein and Albumin 103 Methods of Protein Estimation 103 Determination of Albumin 104 Questions 105 Reagent Preparation 106 16. Determination of Total Cholesterol 107 Cholesterol 107 Questions 109 Reagent Preparation 109 17. Determination of Uric Acid 110 Uric Acid 110 Questions 112 Reagent Preparation 112 18. Determination of Bilirubin 114 Methods 114 Interpretation 116 Jaundice 117 Questions 117 Reagent Preparation 117 19. Determination of Transaminases 118 Transaminases 118 Questions 122 Reagent Preparation 122 20. Determination of Alkaline Phosphatase 124 Methods 124 Isoenzymes of Alkaline Phosphatase 126 Questions 126 Reagent Preparation 126 21. Determination of Total Calcium 128 Calcium 128 Questions 131 Reagent Preparation 132 22. Determination of Phosphate 133 Questions 136 Reagent Preparation 136 Prelims.indd 15 17-07-2015 14:15:34 xvi Practical Biochemistry SECTION 3: CHARTS 23. Charts 141 Acid Base Disorders 141 Jaundice 145 Diabetes Mellitus 149 Inborn Errors of Metabolism 155 Porphyrias 161 Vitamins and Minerals 163 Tumor Markers 166 Water and Electrolytes 167 Kidney Diseases 168 Biochemical Diagnosis of Myocardial Infarction 170 Enzymology 172 SECTION 4: SPOTTERS 24. Spotters 177 Instruments 177 Reagents 183 Indicators 185 Crystals 186 Separation Techniques 188 Graphs 191 Tests 195 Nutrition 203 Spectroscopy 207 Conceptual Questions 208 SECTION 5: OBJECTIVE STRUCTURED PRACTICAL EXAMINATION 25. Objective Structured Practical Examination: Model Questions 221 General Guidelines 221 Index 241 Prelims.indd 16 17-07-2015 14:15:34